02514nas a2200229 4500008004100000245012400041210006900165300001400234490000700248520177800255653001902033653001502052653001502067653001802082653001502100653002802115100002102143700002202164700002202186700002502208856005102233 2019 eng d00aPhylogenomics and mitochondrial genome evolution of the gall-associated doryctine wasp genera (Hymenoptera: Braconidae)0 aPhylogenomics and mitochondrial genome evolution of the gallasso a731–7440 v173 a

The braconid subfamily Doryctinae is a cosmopolitan, highly diverse group of wasps mainly represented by parasitoid species. In this subfamily, however, there is a group of mainly Neotropical genera whose species are associated with galls of several vascular plant families. Previous molecular phylogenetic studies based on a few markers consistently recovered the monophyly of this group, though various relationships among genera and the monophyly of its most speciose genus, Allorhogas Gahan, remained unresolved. We characterized the mitogenomes of 13 representative species of the gall-associated doryctine clade and performed a phylogenomic analysis with both mitogenome and ultraconserved element (UCE) DNA sequence data to assess the classification of Doryctinae and to examine the evolution of phytophagy within the group. We found different patterns of tRNA gene rearrangements, two of which are present in most of the ingroup taxa. All phylogenetic analyses yielded highly similar, well-supported topologies that are congruent with relationships reported in previous studies. The resulting phylogenies confirmed Percnobracon as sister to the remaining genera and supported two separate clades whose genera are exclusively associated with Ficus (Moraceae). Allorhogas is confirmed to be polyphyletic. The combined evidence also supported a main Allorhogas clade composed of multiple phytophagous species that feed on at least five different host plant families. Further rearing records and phylogenetic studies are needed to examine host plant shifts and to broaden our understanding of a poorly studied radiation of gall-associated wasps.

10aIchneumonoidea10amitogenome10aNeotropics10aphylogenomics10aphytophagy10aultraconserved elements1 aSamaca-Saenz, E.1 aMeza-Lazaro, R.N.1 aBranstetter, M.G.1 aZaldivar-Riveron, A. uhttps://doryctinaekey.myspecies.info/node/193403256nas a2200121 4500008004100000245007800041210007000119260007100189490001100260520274900271100002503020856008903045 2014 eng d00aTaxonomia de Doryctinae (Hymenoptera: Braconidae) na Amazônia brasileira0 aTaxonomia de Doryctinae Hymenoptera Braconidae na Amazônia brasi aManaus, BrazilbInstituto Nacional de Pesquisas da Amazôniac20140 vMaster3 a

Braconidae has about 19,500 valid species, and Doryctinae is one of its most diverse subfamilies with over 1000 species in about 180 genera. However, the authors point out that the greater diversity of the group lies in the Neotropics, where there are about 70% of the genera. In Brazil, have been recorded near 60 genera, 32 for the Brazilian Amazon. However, these genera are known only by their type-localities, and their identification is so hardy due to the lack of keys for the region, and the ones that exist belongs to other regions and are outdated. Thus, the objective here was to conduct a taxonomic study of the Doryctinae genera occurring in the Brazilian Amazon. For its realization, mainly material from Instituto Nacional de Pesquisas da Amazônia (INPA) and Museu Paraense Emílio Goeldi (MPEG) were analyzed, besides other entomological collections of the Amazon region. The material was identified in part through the keys of Marsh (1997 and 2002) to the New World and Costa Rica, respectively and original descriptions. It was possible to analyze more than 900 specimens classified into 44 existing genera, beyond recognition of seven new genera and three new species, all here described. Barbalhoa Marsh, Curtiselloides Marsh, Pannuceus Marsh, Percnobracon Kieffer, Vanderentiellus Marsh and Bolivar Zaldívar-Riverón & Rodríguez-Jiménez are new records for Brazil, while Aphelopsia Marsh, Neoheterospilus Belokobylskij, Panama Marsh and Ptesimogastroides Braet & van Achterberg are news for the Brazilian Amazon region; besides 24 genera had their records expanded within this region. The subfamily was recorded in almost all the states that comprise the Brazilian Amazon, except for Tocantins and Mato Grosso. The largest number of genera was found in Amazonas (44), followed by Pará (23) and Roraima (20). It is suggested that further collection efforts should be concentrated on those states that had fewer genera registered (Rondônia, Mato Grosso, Tocantins and Maranhão) and also have greater risk of loss of biodiversity due to deforestation. The taxonomic analysis suggests the existence of a possible synonymy of Johnsonius Marsh under Semirhytus Szépligeti, and Aphelopsia Marsh under Callihormius Ashmead. The Notiospathius Matthews & Marsh and Allorhogas Gahan have a great diversity within the region and should be studied separately in the future, considering the possibility of discovering new species in the first, and a better definition of it limits in the second.

1 ade Souza Gadelha, S. uhttp://w2.files.scire.net.br/atrio/inpa-ent_upl/THESIS/91/dissertao__siangadelha.pdf03170nas a2200217 4500008004100000245013800041210006900179520246000248653001902708653001002727653000902737653000902746100002502755700001902780700002402799700002102823700001502844700001702859700002502876856005102901 2014 eng d00aSystematics and evolution of gall formation in the plant-associated genera of the wasp subfamily Doryctinae (Hymenoptera: Braconidae)0 aSystematics and evolution of gall formation in the plantassociat3 a
Gall formation is a specialised form of phytophagy that consists of abnormal growth of host plant tissue induced by other organisms, principally insects and mites. In the mainly parasitoid wasp subfamily Doryctinae, gall association, represented by gall inducers, inquilines and their parasitoids, is known for species of seven genera. Previous molecular studies recovered few species of six of these genera as monophyletic despite their disparate morphologies. Here, we reconstructed the evolutionary relationships among 47 species belonging to six gall-associated doryctine genera based on two mitochondrial and two nuclear gene markers. Most of the Bayesian analyses, performed with different levels of incomplete taxa and characters, supported the monophyly of gall-associated doryctines, with Heterospilus (Heterospilini) as sister group. Percnobracon Kieffer and Jörgensen and Monitoriella Hedqvist were consistently recovered as monophyletic, and the validity of the monotypic Mononeuron was confirmed with respect to Allorhogas Gahan. A nonmonophyletic Allorhogas was recovered, although without significant support. The relationships obtained and the gathered morphological and biological information led us to erect three new genera originally assigned to PsenobolusFicobolus gen.n. (F. paniaguai sp.n. and F. jaliscoi sp.n.), Plesiopsenobolus gen.n. (Pl. mesoamericanus sp.n., Pl. plesiomorphus van Achterberg and Marsh comb.n., and Pl. tico sp.n.), and Sabinita gen.n. (S. mexicana sp.n.). The origin of the gall-associated doryctine clade was estimated to have occurred during the middle Miocene to early Oligocene, 16.33–30.55 Ma. Our results support the origin of true gall induction in the Doryctinae from parasitoidism of other gall-forming insects. Moreover, adaptations to attack different gall-forming taxa on various unrelated plant families probably triggered species diversification in the main Allorhogas clade and may also have promoted the independent origin of gall formation on at least three plant groups. Species diversification in the remaining doryctine taxa was probably a result of host shifts within a particular plant taxon and shifts to different plant organs.
10aage estimation10aclade10agall10akeys1 aZaldivar-Riveron, A.1 aMartinez, J.J.1 aBelokobylskij, S.A.1 aPedraza-Lara, C.1 aShaw, S.R.1 aHanson, P.E.1 aVarela-Hernandez, F. uhttps://doryctinaekey.myspecies.info/node/182200329nam a2200121 4500008004100000245003000041210002800071300001400099490000700113100002000120700001600140856005100156 1976 eng d00aBraconidae 9. Doryctinae.0 aBraconidae 9 Doryctinae a1263-14240 v131 aShenefelt, R.D.1 aMarsh, P.M. uhttps://doryctinaekey.myspecies.info/node/177000355nas a2200121 4500008004100000245004400041210004300085300001200128490000600140100001800146700001800164856005100182 1910 eng d00aGallen und Gallentiere aus Argentinien.0 aGallen und Gallentiere aus Argentinien a362-4420 v21 aKieffer, J.J.1 aJorgensen, P. uhttps://doryctinaekey.myspecies.info/node/148902729nas a2200217 4500008004100000245013100041210006900172300001200241490000800253520202000261653002002281653002202301653002202323653001102345653002002356653001602376100002402392700002502416700001902441856005102460 2004 eng d00aPhylogeny of the genera of the parasitic wasps subfamily Doryctinae (Hymenoptera: Braconidae) based on morphological evidence.0 aPhylogeny of the genera of the parasitic wasps subfamily Dorycti a369-4040 v1423 a

The evolutionary relationships among most (143 genera) of the currently recognized genera of the braconid wasp subfamily Doryctinae were investigated using maximum parsimony analysis, employing 100 characters from external morphology and four additional, less well-studied character systems (male genitalia, ovipositor structure, venom apparatus and larval cephalic structure). We investigated the ‘performance’ of characters from external morphology and the other character systems and the effects of abundant missing entries by comparing the data decisiveness, retention and consistency indices of four different character partitions. The results indicate that the performances of the different partitions are not related to the proportions of missing entries, but instead are negatively correlated to their proportion of informative characters, suggesting that the morphological information in this group is subject to high levels of homoplasy. The external morphological partition is significantly incongruent with respect to a data set comprising the other character systems based on the ILD test. Analyses supported neither the monophyly of the large tribes Doryctini and Hecabolini, nor the monophyly of the Spathiini and Heterospilini. Relationships obtained from successive approximation weighting analysis for the complete data differ considerably from the currently accepted tribal and subtribal classifications. The only exceptions were the Ypsistocerini and the Ecphylini, whose recognized members were recovered in single clades. A close relationship between the Binaerini and Holcobraconini, and also Monarea, is consistently supported by venom apparatus and ovipositor structure characters but is not indicated by external morphological data. Low bootstrap values obtained for most of the recovered clades in all analyses do not allow us to propose a meaningful reclassification for the group at this time. A complete list of the recognized genera and their synonymies is given.

10aclassification10aconsistency index10adata decisiveness10ainsect10aretention index10asystematics1 aBelokobylskij, S.A.1 aZaldivar-Riveron, A.1 aQuicke, D.L.J. uhttps://doryctinaekey.myspecies.info/node/102501912nas a2200193 4500008004100000245013300041210006900174300001200243490000700255520128200262653000801544653000801552100002501560700002401585700002201609700001901631700001901650856004901669 2008 eng d00aMolecular phylogeny and historical biogeography of the cosmopolitan parasitic wasp subfamily Doryctinae (Hymenoptera:Braconidae)0 aMolecular phylogeny and historical biogeography of the cosmopoli a345-3630 v223 a

The phylogenetic relationships among representatives of 64 genera of the cosmopolitan parasitic wasps of the subfamily Doryctinae were investigated based on nuclear 28S ribosomal (r) DNA (~650 bp of the D2–3 region) and cytochrome c oxidase I (COI) mitochondrial (mt) DNA (603 bp) sequence data. The molecular dating of selected clades and the biogeography of the subfamily were also inferred. The partitioned Bayesian analyses did not recover a monophyletic Doryctinae, though the relationships involved were only weakly supported. Strong evidence was found for rejecting the monophylies of both Doryctes Haliday, 1836 and Spathius Nees, 1818. Our results also support the recognition of the Rhaconotini as a valid tribe. A dispersal–vicariance analysis showed a strong geographical signal for the taxa included, with molecular dating estimates for the origin of Doryctinae and its subsequent radiation both occurring during the late Paleocene–early Eocene. The divergence time estimates suggest that diversification in the subfamily could have in part occurred as a result of continental break-up events that took place in the southern hemisphere, though more recent dispersal events account for the current distribution of several widespread taxa.

10a28S10aCOI1 aZaldivar-Riveron, A.1 aBelokobylskij, S.A.1 aLeon-Regagnon, V.1 aBriceño, R.G.1 aQuicke, D.L.J. uhttps://doryctinaekey.myspecies.info/node/4302111nam a2200145 4500008004100000022001500041245006800056210006200124260005400186300000800240490000700248520164500255100001601900856004901916 2002 eng d a188798814900aThe Doryctinae of Costa Rica (excluding the genus Heterospilus)0 aDoryctinae of Costa Rica excluding the genus Heterospilus aGainsville, Fl.bAmerican Entomological Institute a3190 v703 a

A comprehensive taxonomic study is presented for the 63 genera and 172 species of braconid wasp subfamily Doryctine occurring in Costa Rica. Of these, 12 genera and 119 species are described as new to science. The speciose genus Heterospilus is excluded from this study. Keys for identification of the genera and species are provided and each genus and species is described and illustrated. Several genera previously describe from South America or other parts of Central America are recorded for the first time from Costa Rica. One new synonymy is proposed: the genus Achterbergia Marsh is considered a junior synonym of the genus Aivalykus Nixon (n.syn.). Two new combinations are proposed: Achterbergia arawak Marsh is transferred to Aivalykus (n. comb.); Platydoryctes amazonensis Barbalho and Penteado-Dias is transferred to the new genus Angelica (n.comb.). Very little is known about the biology of doryctine wasps,but where host records are available most species appear to be idiobiont ectoparasitoids of wood boring beetle larvae. However, three genera in Costa Rica depart remarkably from this biology: several species of Allorhogas and Labania have been reared from leaf and seed galls on various plant, and species of Psenobolus develop as inquilines in figs. A checklist of the Costa Rican Doryctinae is provided. A Spanish translation of the key to genera is included as an appendix.

1 aMarsh, P.M. uhttps://doryctinaekey.myspecies.info/node/40