02531nas a2200205 4500008004100000245015300041210006900194300001200263490000800275520183400283653001502117653001402132653002402146653001602170653001502186100002402201700002402225700002502249856005102274 2019 eng d00aMolecular phylogenetics and evolution of generic diagnostic morphological features in the doryctine wasp tribe Rhaconotini (Hymenoptera: Braconidae)0 aMolecular phylogenetics and evolution of generic diagnostic morp a164-1710 v2793 a

The doryctine tribe Rhaconotini (Hymenoptera: Braconidae) is a cosmopolitan group of parasitoid wasps that currently comprises 13 valid genera. Despite the extensive taxonomic studies carried out for this tribe, its generic limits are still unclear due to the considerable variation of its diagnostic morphological features. Here we assessed the evolutionary relationships among representative species of eight rhaconotine genera based on mitochondrial and nuclear DNA sequence data. We also performed ancestral character reconstruction analyses of morphological features that are diagnostic at the genus level and assessed the times of origin and main diversification events in the tribe. The Rhaconotini was significantly supported as monophyletic. The widely distributed, speciose genus Rhaconotus Ruthe as well as Ipodoryctes Granger were recovered as non-monophyletic. Rhaconotus species with five visible metasomal tergites were nested in a single clade with the monotypic Aptenobracon Marsh, which also has this morphological feature. Most Rhaconotus species with six or more visible tergites were intermingled with other Oriental and Afrotropical genera that share this condition. The ancestral character reconstruction analyses of the remaining morphological features showed that they probably were gained and lost repeatedly during the evolution of the group. The origin and early diversification events in the Rhaconotini were estimated to occur during the late Eocene to mid-Miocene. Our results highlight the importance of assessing the evolution of diagnostic morphological features to improve the supraspecific classification of insect taxa with extensive morphological homoplasy.

10aBraconidae10aHomoplasy10amolecular phylogeny10aRhaconotini10aRhaconotus1 aJasso-Martinez, J.M1 aBelokobylskij, S.A.1 aZaldivar-Riveron, A. uhttps://doryctinaekey.myspecies.info/node/194102514nas a2200229 4500008004100000245012400041210006900165300001400234490000700248520177800255653001902033653001502052653001502067653001802082653001502100653002802115100002102143700002202164700002202186700002502208856005102233 2019 eng d00aPhylogenomics and mitochondrial genome evolution of the gall-associated doryctine wasp genera (Hymenoptera: Braconidae)0 aPhylogenomics and mitochondrial genome evolution of the gallasso a731–7440 v173 a

The braconid subfamily Doryctinae is a cosmopolitan, highly diverse group of wasps mainly represented by parasitoid species. In this subfamily, however, there is a group of mainly Neotropical genera whose species are associated with galls of several vascular plant families. Previous molecular phylogenetic studies based on a few markers consistently recovered the monophyly of this group, though various relationships among genera and the monophyly of its most speciose genus, Allorhogas Gahan, remained unresolved. We characterized the mitogenomes of 13 representative species of the gall-associated doryctine clade and performed a phylogenomic analysis with both mitogenome and ultraconserved element (UCE) DNA sequence data to assess the classification of Doryctinae and to examine the evolution of phytophagy within the group. We found different patterns of tRNA gene rearrangements, two of which are present in most of the ingroup taxa. All phylogenetic analyses yielded highly similar, well-supported topologies that are congruent with relationships reported in previous studies. The resulting phylogenies confirmed Percnobracon as sister to the remaining genera and supported two separate clades whose genera are exclusively associated with Ficus (Moraceae). Allorhogas is confirmed to be polyphyletic. The combined evidence also supported a main Allorhogas clade composed of multiple phytophagous species that feed on at least five different host plant families. Further rearing records and phylogenetic studies are needed to examine host plant shifts and to broaden our understanding of a poorly studied radiation of gall-associated wasps.

10aIchneumonoidea10amitogenome10aNeotropics10aphylogenomics10aphytophagy10aultraconserved elements1 aSamaca-Saenz, E.1 aMeza-Lazaro, R.N.1 aBranstetter, M.G.1 aZaldivar-Riveron, A. uhttps://doryctinaekey.myspecies.info/node/193402729nas a2200217 4500008004100000245013100041210006900172300001200241490000800253520202000261653002002281653002202301653002202323653001102345653002002356653001602376100002402392700002502416700001902441856005102460 2004 eng d00aPhylogeny of the genera of the parasitic wasps subfamily Doryctinae (Hymenoptera: Braconidae) based on morphological evidence.0 aPhylogeny of the genera of the parasitic wasps subfamily Dorycti a369-4040 v1423 a

The evolutionary relationships among most (143 genera) of the currently recognized genera of the braconid wasp subfamily Doryctinae were investigated using maximum parsimony analysis, employing 100 characters from external morphology and four additional, less well-studied character systems (male genitalia, ovipositor structure, venom apparatus and larval cephalic structure). We investigated the ‘performance’ of characters from external morphology and the other character systems and the effects of abundant missing entries by comparing the data decisiveness, retention and consistency indices of four different character partitions. The results indicate that the performances of the different partitions are not related to the proportions of missing entries, but instead are negatively correlated to their proportion of informative characters, suggesting that the morphological information in this group is subject to high levels of homoplasy. The external morphological partition is significantly incongruent with respect to a data set comprising the other character systems based on the ILD test. Analyses supported neither the monophyly of the large tribes Doryctini and Hecabolini, nor the monophyly of the Spathiini and Heterospilini. Relationships obtained from successive approximation weighting analysis for the complete data differ considerably from the currently accepted tribal and subtribal classifications. The only exceptions were the Ypsistocerini and the Ecphylini, whose recognized members were recovered in single clades. A close relationship between the Binaerini and Holcobraconini, and also Monarea, is consistently supported by venom apparatus and ovipositor structure characters but is not indicated by external morphological data. Low bootstrap values obtained for most of the recovered clades in all analyses do not allow us to propose a meaningful reclassification for the group at this time. A complete list of the recognized genera and their synonymies is given.

10aclassification10aconsistency index10adata decisiveness10ainsect10aretention index10asystematics1 aBelokobylskij, S.A.1 aZaldivar-Riveron, A.1 aQuicke, D.L.J. uhttps://doryctinaekey.myspecies.info/node/102502113nas a2200277 4500008004100000022001400041245011700055210006900172300001000241490000700251520127200258653001501530653000901545653001801554653001501572653001601587653002401603100002501627700001901652700002101671700002701692700002601719700002401745700001601769856005001785 2010 eng d a1940-173600aDNA barcoding a highly diverse group of parasitoid wasps (Braconidae: Doryctinae) from a Mexican nature reserve.0 aDNA barcoding a highly diverse group of parasitoid wasps Braconi a18-230 v213 a

Background and aims. The preliminary results of a DNA barcoding study of the doryctine fauna of parasitoid wasps from the Chamela–Cuixmala Biosphere Reserve in Mexico, a region dominated by tropical dry forest, are presented. So far, three field trips have been carried out to the reserve and 468 specimens have been collected, of which 407 cox1 sequences were obtained.
Materials and methods. The general mixed Yule-coalescent model was applied to a phylogram to investigate the number of evolutionary units that can be detected from the DNA sequence data examined.
Results. A total of 185 barcoding species assigned to 20 identified doryctine genera were discriminated using the above model, 115 of which belong to the speciose genus Heterospilus, pointing out the extraordinary species richness of this subfamily of insects in a Mexican tropical dry forest.
Conclusion. On the basis of the DNA barcodes generated, Ptesimogastroides Braet & van Achterberg is proposed to be a junior synonym of Ptesimogaster Marsh syn. nov. Neoheterospilus was also found deeply nested within a large Heterospilus clade, suggesting the paraphyly of the latter genus.

10aBraconidae10acox110aDNA barcoding10aDoryctinae10aHymenoptera10atropical dry forest1 aZaldivar-Riveron, A.1 aMartinez, J.J.1 aCeccarelli, F.S.1 aDe Jesus-Bonilla, V.S.1 aRodriguez-Perez, A.C.1 aResendiz-Flores, A.1 aSmith, M.A. uhttps://doryctinaekey.myspecies.info/node/93301912nas a2200193 4500008004100000245013300041210006900174300001200243490000700255520128200262653000801544653000801552100002501560700002401585700002201609700001901631700001901650856004901669 2008 eng d00aMolecular phylogeny and historical biogeography of the cosmopolitan parasitic wasp subfamily Doryctinae (Hymenoptera:Braconidae)0 aMolecular phylogeny and historical biogeography of the cosmopoli a345-3630 v223 a

The phylogenetic relationships among representatives of 64 genera of the cosmopolitan parasitic wasps of the subfamily Doryctinae were investigated based on nuclear 28S ribosomal (r) DNA (~650 bp of the D2–3 region) and cytochrome c oxidase I (COI) mitochondrial (mt) DNA (603 bp) sequence data. The molecular dating of selected clades and the biogeography of the subfamily were also inferred. The partitioned Bayesian analyses did not recover a monophyletic Doryctinae, though the relationships involved were only weakly supported. Strong evidence was found for rejecting the monophylies of both Doryctes Haliday, 1836 and Spathius Nees, 1818. Our results also support the recognition of the Rhaconotini as a valid tribe. A dispersal–vicariance analysis showed a strong geographical signal for the taxa included, with molecular dating estimates for the origin of Doryctinae and its subsequent radiation both occurring during the late Paleocene–early Eocene. The divergence time estimates suggest that diversification in the subfamily could have in part occurred as a result of continental break-up events that took place in the southern hemisphere, though more recent dispersal events account for the current distribution of several widespread taxa.

10a28S10aCOI1 aZaldivar-Riveron, A.1 aBelokobylskij, S.A.1 aLeon-Regagnon, V.1 aBriceño, R.G.1 aQuicke, D.L.J. uhttps://doryctinaekey.myspecies.info/node/43