@article {1761, title = {Broad polyphyly and historical biogeography of the neotropical wasp genus Notiospathius (Braconidae: Doryctinae)}, journal = {Molecular Phylogenetics and Evolution}, volume = {69}, year = {2013}, pages = {142-152}, abstract = {

Morphological convergence is a frequently observed pattern that occurs in species with similar environments,\ trophic niches, and/or life history strategies. In particular, adaptive morphological convergence is\ common in small, highly diverse invertebrate taxa sharing similar life history strategies such as parasitoidism.\ The genus Notiospathius is a braconid parasitoid wasp group with a vast, undetermined number\ of species distributed throughout the Neotropics. Members of this genus possess a considerably elongated,\ petiolate first metasomal tergite. This structure, however, also occurs in species of other doryctine\ genera, both closely and distantly related, probably due to adaptation for attacking wood and bark boring\ coleopteran larvae. Here we investigated the phylogenetic relationships among 117 species assigned to\ Notiospathius, including species of its closely related genera Masonius and Tarasco, as well as members\ of a number of other doryctine genera in order to test its monophyly. Separate and concatenated Bayesian\ partitioned analyses were carried out using two mitochondrial and three nuclear gene markers. The influence\ of each gene on the overall topology was verified using a cross-validation analysis for each marker\ with a \‘\‘leave-one-out\’\’ approach. Moreover, the historical biogeography of Notiospathius was assessed\ calculating divergence time estimates of clades with BEAST and performing ancestral area reconstruction\ analyses both with RASP (Bayesian and S-DIVA) and Lagrange (DEC). All analyses recovered a polyphyletic\ Notiospathius consisting of three separate clades that were more related to other doryctine genera\ than to each other. The relationships reconstructed could not be used to confidently establish the limits\ of Notiospathius with respect to its closely related genera Tarasco and Masonius, and thus the generic status\ of the latter two taxa is maintained. Our divergence time estimates and ancestral area reconstructions\ indicate that the most recent common ancestor of Notiospathius sensu stricto originated in the Caribbean\ subregion during the Oligocene\–Miocene boundary, and subsequently diversified during the mid- to late\ Miocene, radiating throughout the Neotropics.

}, keywords = {concatened analysis, Hymenoptera, Ichneumonoidea, parasitoid, phylogeny}, doi = {10.1016/j.ympev.2013.05.001}, author = {Ceccarelli, F.S. and A. Zaldivar-Riveron} } @article {1634, title = {The genus Iare Barbalho and Penteado-Dias (Hymenoptera: Braconidae: Doryctinae) in Mexico, with the description of two new species.}, journal = {Zootaxa}, volume = {2685}, year = {2010}, pages = {30-38}, author = {J.J. Martinez and Ceccarelli, F.S. and A. Zaldivar-Riveron} } @article {932, title = {Five new species of the genera Heerz Marsh, Lissopsius Marsh and Ondigus Braet, Barbalho and van Achterberg (Braconidae, Doryctinae) from the Chamela-Cuixmala biosphere reserve in Jalisco, Mexico.}, journal = {Zookeys}, volume = {164}, year = {2012}, pages = {1-23}, author = {A. Zaldivar-Riveron and J.J. Martinez and Ceccarelli, F.S. and S.R. Shaw} } @article {933, title = {DNA barcoding a highly diverse group of parasitoid wasps (Braconidae: Doryctinae) from a Mexican nature reserve.}, journal = {Mitochondrial DNA}, volume = {21}, year = {2010}, pages = {18-23}, abstract = {

Background and aims. The preliminary results of a DNA barcoding study of the doryctine fauna of parasitoid wasps from the Chamela\–Cuixmala Biosphere Reserve in Mexico, a region dominated by tropical dry forest, are presented. So far, three field trips have been carried out to the reserve and 468 specimens have been collected, of which 407 cox1 sequences were obtained.
Materials and methods. The general mixed Yule-coalescent model was applied to a phylogram to investigate the number of evolutionary units that can be detected from the DNA sequence data examined.
Results. A total of 185 barcoding species assigned to 20 identified doryctine genera were discriminated using the above model, 115 of which belong to the speciose genus Heterospilus, pointing out the extraordinary species richness of this subfamily of insects in a Mexican tropical dry forest.
Conclusion. On the basis of the DNA barcodes generated, Ptesimogastroides Braet \& van Achterberg is proposed to be a junior synonym of Ptesimogaster Marsh syn. nov. Neoheterospilus was also found deeply nested within a large Heterospilus clade, suggesting the paraphyly of the latter genus.

}, keywords = {Braconidae, cox1, DNA barcoding, Doryctinae, Hymenoptera, tropical dry forest}, issn = {1940-1736}, doi = {10.3109/19401736.2010.523701}, author = {A. Zaldivar-Riveron and J.J. Martinez and Ceccarelli, F.S. and V.S. De Jesus-Bonilla and Rodriguez-Perez, A.C. and Resendiz-Flores, A. and Smith, M.A.} } @article {355, title = {Redescription of Mononeuron duguetiae Fischer (Braconidae, Doryctinae), a gall associated species on Duguetia furfuracea (St. Hil.) (Annonaceae).}, journal = {Journal of Hymenoptera Research}, volume = {24}, year = {2012}, pages = {75-84}, author = {J.F. Nunes and A.M. Penteado-Dias and Ceccarelli, F.S. and A. Zaldivar-Riveron} } @article {62, title = {Kauriphanes n. gen., a new genus of braconid parasitoid wasp (Hymenoptera: Braconidae: Doryctinae) from New Zealand}, journal = {Annales de la soci{\'e}t{\'e} entomologique de France}, volume = {47}, year = {2011}, pages = {394-401}, abstract = {

A new genus belonging to the braconid wasp subfamily Doryctinae, Kauriphanes n. gen. (type species K. khalaimi n. sp.), is described from New Zealand. This genus is placed within the doryctine subtribe Caenophanina. The extent of this subtribe is discussed and the phylogenetic relationships of three of its genera were investigated using one mitochondrial and one nuclear DNA sequence markers. Similar to previous studies, the Bayesian analyses performed significantly support a clade with the included members of Caenophanina as a sister group of a clade with the examined species of Spathiini sensu stricto. The placement of the Caenophanini within Doryctini, however, is left pendant to further exhaustive phylogenetic studies. A key to genera and subgenera belonging to Caenophanina is given.

}, keywords = {Caenophanina, mitochondrial DNA, nuclear DNA, phylogeny, Spathiini}, author = {S.A. Belokobylskij and Ceccarelli, F.S. and A. Zaldivar-Riveron} } @article {46, title = {Species identification in the taxonomically neglected, highly diverse, neotropical parasitoid wasp genus Notiospathius (Braconidae: Doryctinae) based on an integrative molecular and morphological approach}, journal = {Molecular Phylogenetics and Evolution}, volume = {62}, year = {2012}, pages = {485-495}, abstract = {

Various DNA sequence-based methods for species delineation have recently been developed to assess the species-richness of highly diverse, neglected invertebrate taxa. These methods, however, need to be tested under a variety of conditions, including the use of different markers and parameters. Here, we explored the species diversity of a species-rich group of braconid parasitoid wasps, the Neotropical genus Notiospathius, including 233 specimens from 10 different countries. We examined sequences of two mitochondrial (mt) (COI, cyt b) and one nuclear (wg) gene fragments. We analysed them separately as well as concatenating the mt data with the general mixed Yule-coalescent (GMYC) model for species delineation using different tree-building methods and parameters for reconstructing ultrametric trees. We evaluated the performance of GMYC analyses by comparing their species delineations with our morphospecies identifications. Reconstructing ultrametric trees with a relaxed lognormal clock rate using the program BEAST gave the most congruent results with morphology for the two mt markers. A tree obtained with wg using the programs MrBayes+Pathd8 had the fewest cases of incongruence with morphology, though the performance of this nuclear marker was considerably lower than that of COI and cyt b. Species delimitation using the coalescent prior to obtain ultrametric trees was morphologically more congruent with COI, whereas the Yule prior was more congruent with cyt b. The analyses concatenating the mt datasets failed to recover some species supported both by morphology and the separate analyses of the mt markers. The highest morphological congruence was obtained with the GMYC analysis on an ultrametric tree reconstructed with cyt b using the relaxed lognormal clock rate and the Yule prior, thus supporting the importance of using alternative markers when the information of the barcoding locus (COI) is not concordant with morphological evidence. Seventy-one species were delimited based on the congruence found among COI, cyt b and morphology. Both mt markers also revealed the existence of seven potential cryptic species. This high species richness from a scattered geographical sampling indicates that there is a remarkable number of Notiospathius species that remains undiscovered.

}, keywords = {Doryctinae, GMYC model, integrative taxonomy, Species delineation}, doi = {10.1016/j.ympev.2011.10.018}, author = {Ceccarelli, F.S. and M.J. Sharkey and A. Zaldivar-Riveron} } @article {45, title = {Seven new species of Notiospathius (Hymenoptera, Braconidae, Doryctinae) from Northwest Venezuela}, journal = {Journal of Hymenoptera Research}, volume = {29}, year = {2012}, pages = {37-61}, abstract = {

We describe seven new species of the doryctine wasp genus Notiospathius Matthews \& Marsh from Northwest Venezuela: N. araguae sp. n., N. bolivari sp. n., N. curvilineatus sp. n., N. dantei sp. n., N. estradae sp. n., N. larensis sp. n., and N. venezuelae sp. n. These represent the first described species of the genus reported for this country. Species boundaries for the above taxa were confirmed using the General mixed Yule-coalescent method with 441 DNA barcode sequences from specimens assigned to Notiospathius and other closely related genera collected in different countries along the Neotropics.

}, keywords = {Barcoding, GMYC model, integrative taxonomy, Neotropical, parasitoid}, doi = {10.3897/JHR.29.3555}, author = {L{\'o}pez-Estrada, E.K. and R.G. Brice{\~n}o and Smith, M.A. and J.F. Nunes and A.M. Penteado-Dias and Ceccarelli, F.S. and Clebsch, H. and A. Zaldivar-Riveron} } @article {37, title = {Two new species of Evaniodes Sz{\'e}pligeti (Hymenoptera: Braconidae: Doryctinae) from French Guiana}, journal = {Zootaxa}, volume = {3247}, year = {2012}, pages = {52-60}, abstract = {
Two new species belonging to the enigmatic doryctine genus Evaniodes Sz\épligeti, 1901 (Hymenoptera: Braconidae), E.\ palikuri Braet, Ceccarelli \& Zald\ívar-River\ón sp. nov. and E. wayampisi Braet, Ceccarelli \& Zald\ívar-River\ón sp. nov., are described from French Guiana. Two of the three previously described species of Evaniodes, E. areolatus Sz\épligeti, 1901 and E. spathiiformis Sz\épligeti, 1901, are recorded for the first time for French Guiana. A key to the five described species of the genus is provided.
}, keywords = {Braconidae, DNA barcoding, Doryctinae, Evaniodes, French Guiana}, issn = {1175-5334}, author = {Braet, Y. and Ceccarelli, F.S. and A. Zaldivar-Riveron} }